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| Cezar,Alfredo Skrebsky; Glanzner,Werner Giehl; Gutierrez,Karina; Anezi-Junior,Paulo Afonso; Nilles,Carlise Reichert; Comim,Fábio Vasconcellos; Vogel,Fernanda Silveira Flores; Gonçalves,Paulo Bayard Dias. |
| Cell therapy has shown encouraging perspectives for human and veterinary medicine. Experimentally, genetic manipulation allows to mark and locate allogeneic cells. However, this makes their genotype/phenotype different from non-marked cells used clinically. Alternatively, the presence of the Y-chromosome enables male donor cells detection in female organisms. However, the concentration of engrafted cells may be minimal in tissues, due to systemic distribution. In this study, a nested-PCR multiplex test was developed, aiming to increase the sensitivity of the presence/absence diagnosis of male mice adipose-derived (ADSC-Y) and bone marrow mononuclear (BMNC-Y) cells in samples of blood and lungs from females, after endovenous transplantation. Four females... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cellular therapy; Transplantation; Allogeneic cells; Diagnosis; Y-chromosome.. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782015000500905 |
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| Rigo,Melânia Lazzari; Dau,Andressa Minussi Pereira; Glanzner,Werner Giehl; Martins,Manoel; Zanella,Renato; Rizzetti,Tiele Medianeira; Comim,Fabio Vasconcellos; Gonçalves,Paulo Bayard Dias. |
| The main objective of this study was to detect the steroidogenic effects of Ang II in bovine theca cells in vitro. Bovine theca cells were obtained from follicles (larger than 10mm of diameter) collected from a local abattoir and submitted to different treatments in a sequence of experiments. In experiment 1, CYP17A1 mRNA profile was evaluated in LH- (10ng ml-1) and Ang II-treated (0.1µM) theca cells. In experiment 2, a dose-response effect of Ang II (0.001; 0.1 e 10µM) plus insulin (100ng ml-1) and LH (100ng ml-1) was evaluated on steroidogenesis of bovine theca cells. Experiment 3 explored the effects of saralasin (an antagonist of Ang II receptors) on steroid production and steroidogenic enzymes regulation in theca cells. After 24 hours, culture media... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: RAS; LH; Steroidogenesis; Ovary. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782015000400704 |
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